fitc soybean agglutinin sba (Vector Laboratories)
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Fitc Soybean Agglutinin Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/FL-1011-2/Fluorescein+labeled+Soybean+Agglutinin+(SBA)/pm42878060-189-17-20
Average 94 stars, based on 33 article reviews
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Whole Genome Amplification:Article Title: Enzymatic tailoring of anionic glycans for characterizing lectin and antibody specificities in a microarray format Article Snippet: β1,3-glucuronyltransferase (B3GAT1), β1,3-galactosyltransferase (B3GalT5), carbohydrate sulfotransferases 1 (CHST1), 2 (CHST2) and 10 (CHST10), galactose-3-O-sulfotransferase 2 (GAL3ST2), β-galactoside α2,3-sialyltransferase 4 (ST3GAL4) and fucosyltransferase 3 (FUT3); all human, were purchased from R&D Systems; α2,8-sialyltransferase from Campylobacter jejuni (CstII) from ChemilyBio; mutant β1,4-galactosyltransferase (Gal-T1 (Y289L)) for N -acetylgalactosamine transfer was part of the Click-IT O-GlcNAc Enzymatic Labeling System from ThermoFisher, and Pasteurella multocida α2,3-sialyltransferase (PmST3) purchased also from Sigma-Aldrich. .. Biotinylated lectins concanavalin A (ConA, 5 mg/ml), Erythrina cristagalli lectin (ECL, 5 mg/ml), Griffonia simplicifolia isolectin B4 (GSL-I, 0.5 mg/ml) and II (GSL-II, 2 mg/ml), Lens culinaris lectin (LCA, 5 mg/ml), Maackia amurensis lectin I and II (MAA-I, 2 mg/ml; MAA-II, 1 mg/ml), peanut lectin (PNA 5 mg/ml), Ricinus communis lectin I (RCA, 5 mg/ml), Sambucus nigra /Elderberry Bark lectin (SNA, 2 mg/ml), Vicia villosa lectin (VVA, 2 mg/ml), Wisteria floribunda agglutinin (WFA, 2 mg/ml) and wheat germ agglutinin (WGA, 5 mg/ml), as well as Article Title: Selectively targeting UDP-glucose 4-epimerase MoUGE1 for controlling rice blast disease. Article Snippet: Stained germinating conidia and appressoria were observed and imaged using a fluorescence microscope (Nikon Ni90, Japan). .. During various developmental stages of M. oryzae, galactosaminogalactan was visualized by staining with 10 lg/mL Article Title: Enzymatic tailoring of anionic glycans for characterising lectin and antibody specificities in a microarray format. Article Snippet: β1,3-glucuronyltransferase (B3GAT1), β1,3-galactosyltransferase (B3GalT5), carbohydrate sulphotransferases 1 (CHST1), 2 (CHST2) and 10 (CHST10), galactose-3-O-sulphotransferase 2 (GAL3ST2), β-galactoside 2,3-sialyltransferase 4 (ST3GAL4) and fucosyltransferase 3 (FUT3); all human, were purchased from R&D Systems, Minneapolis, MN, US; 2,8-sialyltransferase from Campylobacter jejuni (CstII) from ChemilyBio, Peachtree Corners, GA, US; mutant β1,4galactosyltransferase (Gal-T1 (Y289L)) for N-acetylgalactosamine transfer was part of the ClickIT O-GlcNAc Enzymatic Labeling System from ThermoFisher, Waltham, MA, US and Pasteurella multocida 2,3-sialyltransferase (PmST3) purchased also from Sigma-Aldrich. .. Lectins and Primary Antibodies Biotinylated lectins concanavalin A (ConA, 5 mg/ml), Erythrina cristagalli lectin (ECL, 5 mg /ml), Griffonia simplicifolia isolectin B4 (GSL-I, 0.5 mg/ml) and II (GSL-II, 2 mg/ml), Lens culinaris lectin (LCA, 5 mg/ml), Maackia amurensis lectin I and II (MAA-I, 2 mg/ml; MAA-II, 1 mg/ml), peanut lectin (PNA 5 mg/ml), Ricinus communis lectin I (RCA, 5 mg/ml), Sambucus nigra/Elderberry Bark lectin (SNA, 2 mg/ml), Vicia villosa lectin (VVA, 2 mg/ml), Wisteria floribunda agglutinin (WFA, 2 mg/ml) and wheat germ agglutinin (WGA, 5 mg/ml), as well as Article Title: Graph attention network-driven single-cell omics analysis reveals how B4GALNT1-mediated glycosylation remodelling of the ferroptosis-pyroptosis network promotes drug resistance in hepatocellular carcinoma. Article Snippet: .. Fluorescently labelled lectins were used in the experiment, including FITCvicia villosa agglutinin (VVA; Vector Laboratories, FL-1231, USA), Staining:Article Title: Selectively targeting UDP-glucose 4-epimerase MoUGE1 for controlling rice blast disease. Article Snippet: Stained germinating conidia and appressoria were observed and imaged using a fluorescence microscope (Nikon Ni90, Japan). .. During various developmental stages of M. oryzae, galactosaminogalactan was visualized by staining with 10 lg/mL Incubation:Article Title: Selectively targeting UDP-glucose 4-epimerase MoUGE1 for controlling rice blast disease. Article Snippet: Stained germinating conidia and appressoria were observed and imaged using a fluorescence microscope (Nikon Ni90, Japan). .. During various developmental stages of M. oryzae, galactosaminogalactan was visualized by staining with 10 lg/mL Article Title: PAX gene expression in autosomal dominant polycystic kidney disease contributes to cyst expansion and regulates a gene network associated with cyst growth. Article Snippet: .. After blocking in animal-free blocking buffer (Vector Laboratories, SP-5035), sections were incubated with primary antibodies (rabbit monoclonal anti-PAX2 (1:30; Abcam, ab79389), rabbit polyclonal anti-PAX8 (1:100; Biopat, PA 030), and anti-LRP2/megalin (1:200; Abcam, ab76969)), Alexa Fluor 594-conjugated goat anti-rabbit IgG (H + L) secondary antibody (1:200; ThermoFisher, A11012), and FITC-conjugated lectins (Lotus Tetragonolobus lectin (LTL; 1:50; ThermoFisher, L32480) and Article Title: Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in bacteria. Article Snippet: Template DNA for bacterial OSTs was codon optimized and obtained as double-stranded DNA fragments known as gBlocks (Integrated DNA Technologies). .. Library transformants were replicated onto a nitrocellulose transfer membrane (BioRad, 0.45 μm), which were then placed colony-side-up on a second nitrocellulose transfer membrane and incubated at 30 °C for 18 h. The nitrocellulose transfermembranes were washed in Tris-buffered saline (TBS) for 10min, blocked in 5% bovine serum albumin for 30min, and probed for 1 h with Article Title: Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in bacteria Article Snippet: .. Library transformants were replicated onto a nitrocellulose transfer membrane (BioRad, 0.45 μm), which were then placed colony-side-up on a second nitrocellulose transfer membrane and incubated at 30 °C for 18 h. The nitrocellulose transfer membranes were washed in Tris-buffered saline (TBS) for 10 min, blocked in 5% bovine serum albumin for 30 min, and probed for 1 h with Blocking Assay:Article Title: PAX gene expression in autosomal dominant polycystic kidney disease contributes to cyst expansion and regulates a gene network associated with cyst growth. Article Snippet: .. After blocking in animal-free blocking buffer (Vector Laboratories, SP-5035), sections were incubated with primary antibodies (rabbit monoclonal anti-PAX2 (1:30; Abcam, ab79389), rabbit polyclonal anti-PAX8 (1:100; Biopat, PA 030), and anti-LRP2/megalin (1:200; Abcam, ab76969)), Alexa Fluor 594-conjugated goat anti-rabbit IgG (H + L) secondary antibody (1:200; ThermoFisher, A11012), and FITC-conjugated lectins (Lotus Tetragonolobus lectin (LTL; 1:50; ThermoFisher, L32480) and Membrane:Article Title: Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in bacteria. Article Snippet: Template DNA for bacterial OSTs was codon optimized and obtained as double-stranded DNA fragments known as gBlocks (Integrated DNA Technologies). .. Library transformants were replicated onto a nitrocellulose transfer membrane (BioRad, 0.45 μm), which were then placed colony-side-up on a second nitrocellulose transfer membrane and incubated at 30 °C for 18 h. The nitrocellulose transfermembranes were washed in Tris-buffered saline (TBS) for 10min, blocked in 5% bovine serum albumin for 30min, and probed for 1 h with Article Title: Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in bacteria Article Snippet: .. Library transformants were replicated onto a nitrocellulose transfer membrane (BioRad, 0.45 μm), which were then placed colony-side-up on a second nitrocellulose transfer membrane and incubated at 30 °C for 18 h. The nitrocellulose transfer membranes were washed in Tris-buffered saline (TBS) for 10 min, blocked in 5% bovine serum albumin for 30 min, and probed for 1 h with Saline:Article Title: Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in bacteria. Article Snippet: Template DNA for bacterial OSTs was codon optimized and obtained as double-stranded DNA fragments known as gBlocks (Integrated DNA Technologies). .. Library transformants were replicated onto a nitrocellulose transfer membrane (BioRad, 0.45 μm), which were then placed colony-side-up on a second nitrocellulose transfer membrane and incubated at 30 °C for 18 h. The nitrocellulose transfermembranes were washed in Tris-buffered saline (TBS) for 10min, blocked in 5% bovine serum albumin for 30min, and probed for 1 h with Article Title: Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in bacteria Article Snippet: .. Library transformants were replicated onto a nitrocellulose transfer membrane (BioRad, 0.45 μm), which were then placed colony-side-up on a second nitrocellulose transfer membrane and incubated at 30 °C for 18 h. The nitrocellulose transfer membranes were washed in Tris-buffered saline (TBS) for 10 min, blocked in 5% bovine serum albumin for 30 min, and probed for 1 h with |

