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fitc soybean agglutinin sba  (Vector Laboratories)


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    Structured Review

    Vector Laboratories fitc soybean agglutinin sba
    Fitc Soybean Agglutinin Sba, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/FL-1011-2/Fluorescein+labeled+Soybean+Agglutinin+(SBA)/pm42878060-189-17-20
    Average 94 stars, based on 33 article reviews
    fitc soybean agglutinin sba - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Whole Genome Amplification:

    Article Title: Enzymatic tailoring of anionic glycans for characterizing lectin and antibody specificities in a microarray format
    Article Snippet: β1,3-glucuronyltransferase (B3GAT1), β1,3-galactosyltransferase (B3GalT5), carbohydrate sulfotransferases 1 (CHST1), 2 (CHST2) and 10 (CHST10), galactose-3-O-sulfotransferase 2 (GAL3ST2), β-galactoside α2,3-sialyltransferase 4 (ST3GAL4) and fucosyltransferase 3 (FUT3); all human, were purchased from R&D Systems; α2,8-sialyltransferase from Campylobacter jejuni (CstII) from ChemilyBio; mutant β1,4-galactosyltransferase (Gal-T1 (Y289L)) for N -acetylgalactosamine transfer was part of the Click-IT O-GlcNAc Enzymatic Labeling System from ThermoFisher, and Pasteurella multocida α2,3-sialyltransferase (PmST3) purchased also from Sigma-Aldrich. .. Biotinylated lectins concanavalin A (ConA, 5 mg/ml), Erythrina cristagalli lectin (ECL, 5 mg/ml), Griffonia simplicifolia isolectin B4 (GSL-I, 0.5 mg/ml) and II (GSL-II, 2 mg/ml), Lens culinaris lectin (LCA, 5 mg/ml), Maackia amurensis lectin I and II (MAA-I, 2 mg/ml; MAA-II, 1 mg/ml), peanut lectin (PNA 5 mg/ml), Ricinus communis lectin I (RCA, 5 mg/ml), Sambucus nigra /Elderberry Bark lectin (SNA, 2 mg/ml), Vicia villosa lectin (VVA, 2 mg/ml), Wisteria floribunda agglutinin (WFA, 2 mg/ml) and wheat germ agglutinin (WGA, 5 mg/ml), as well as fluorescein-labeled soybean lectin (SBA, 2 mg/ml) were purchased from Vector Laboratories. .. Donor substrates 3′-phosphoadenosine-5′-phosphosulfate (PAPS), cytidine 5′-monophosphate- N -acetyl-β-neuraminic acid (CMP-Neu5Ac), cytidine 5′-monophosphate- N- glycolyl-β-neuraminic acid disodium salt (CMP-Neu5Gc), guanosine 5′-diphospho-β-L-fucose disodium salt (GDP-Fuc), uridine-5-diphospho- N -acetyl-α-galactosamine sodium salt (UDP-GalNAc), uridine-5-diphospho-α-glucuronic acid trisodium salt (UDP-GlcA), uridine-5-diphospho-α-galactose (UDP-Gal) were purchased from R&D Systems (PAPS), Jennewein Biotechnologie GmbH (CMP-Neu5Ac and GDP-Fuc); ChemilyBio (CMP-Neu5Gc) and Sigma-Aldrich (all others) respectively.

    Article Title: Selectively targeting UDP-glucose 4-epimerase MoUGE1 for controlling rice blast disease.
    Article Snippet: Stained germinating conidia and appressoria were observed and imaged using a fluorescence microscope (Nikon Ni90, Japan). .. During various developmental stages of M. oryzae, galactosaminogalactan was visualized by staining with 10 lg/mL SBA conjugated with Fluorescein (FL-1011–2, Vector Labs, USA) for 30 min. Chitin detection was performed by staining with 10 mg/ ml WGA conjugated with Alexa Fluor 350 (Invitrogen) for 30 min. For invasive hyphae staining, conidial suspensions were dropped onto barley leaves and incubated under high humidity. .. At 30 hpi, the conidial droplets were treated with SBA with Fluorescein or WGA and observed after an additional 30 min. Stained samples were examined under a confocal laser scanning microscope (Leica SP8, Leica Microsystems, Germany) to assess the distribution and changes of these components during M. oryzae development.

    Article Title: Enzymatic tailoring of anionic glycans for characterising lectin and antibody specificities in a microarray format.
    Article Snippet: β1,3-glucuronyltransferase (B3GAT1), β1,3-galactosyltransferase (B3GalT5), carbohydrate sulphotransferases 1 (CHST1), 2 (CHST2) and 10 (CHST10), galactose-3-O-sulphotransferase 2 (GAL3ST2), β-galactoside 2,3-sialyltransferase 4 (ST3GAL4) and fucosyltransferase 3 (FUT3); all human, were purchased from R&D Systems, Minneapolis, MN, US; 2,8-sialyltransferase from Campylobacter jejuni (CstII) from ChemilyBio, Peachtree Corners, GA, US; mutant β1,4galactosyltransferase (Gal-T1 (Y289L)) for N-acetylgalactosamine transfer was part of the ClickIT O-GlcNAc Enzymatic Labeling System from ThermoFisher, Waltham, MA, US and Pasteurella multocida 2,3-sialyltransferase (PmST3) purchased also from Sigma-Aldrich. .. Lectins and Primary Antibodies Biotinylated lectins concanavalin A (ConA, 5 mg/ml), Erythrina cristagalli lectin (ECL, 5 mg /ml), Griffonia simplicifolia isolectin B4 (GSL-I, 0.5 mg/ml) and II (GSL-II, 2 mg/ml), Lens culinaris lectin (LCA, 5 mg/ml), Maackia amurensis lectin I and II (MAA-I, 2 mg/ml; MAA-II, 1 mg/ml), peanut lectin (PNA 5 mg/ml), Ricinus communis lectin I (RCA, 5 mg/ml), Sambucus nigra/Elderberry Bark lectin (SNA, 2 mg/ml), Vicia villosa lectin (VVA, 2 mg/ml), Wisteria floribunda agglutinin (WFA, 2 mg/ml) and wheat germ agglutinin (WGA, 5 mg/ml), as well as fluorescein labelled soybean lectin (SBA, 2 mg/ml) were purchased from Vector Laboratories, Newark, CA, US. .. Donor substrates 3'- phosphoadenosine-5'-phosphosulphate (PAPS), cytidine 5′-monophosphate-N-acetyl-- neuraminic acid (CMP-Neu5Ac), cytidine 5′-monophosphate-N-glycolyl--neuraminic acid disodium salt (CMP-Neu5Gc), guanosine 5'-diphospho--L-fucose disodium salt (GDP-Fuc), uridine-5-diphospho-N-acetyl--galactosamine sodium salt (UDP-GalNAc), uridine-5- Jo urn al Pr e-p roo f 10 diphospho--glucuronic acid trisodium salt (UDP-GlcA), uridine-5-diphospho--galactose (UDP-Gal) were purchased from R&D Systems (PAPS), Jennewein Biotechnologie GmbH, Rheinbreitbach, Germany (CMP-Neu5Ac and GDP-Fuc); ChemilyBio (CMP-Neu5Gc) and SigmaAldrich (all others) respectively.

    Article Title: Graph attention network-driven single-cell omics analysis reveals how B4GALNT1-mediated glycosylation remodelling of the ferroptosis-pyroptosis network promotes drug resistance in hepatocellular carcinoma.
    Article Snippet: .. Fluorescently labelled lectins were used in the experiment, including FITCvicia villosa agglutinin (VVA; Vector Laboratories, FL-1231, USA), FITC-soybean agglutinin (SBA) (Vector Laboratories, FL-1011, USA), Alexa Fluor 488-labelled wheat germ agglutinin (WGA; ThermoFisher Scientific,W11261, USA), and FITC-Ricinus Communis Agglutinin I (RCA-I) (Vector Laboratories, FL-1081, USA). ..

    Staining:

    Article Title: Selectively targeting UDP-glucose 4-epimerase MoUGE1 for controlling rice blast disease.
    Article Snippet: Stained germinating conidia and appressoria were observed and imaged using a fluorescence microscope (Nikon Ni90, Japan). .. During various developmental stages of M. oryzae, galactosaminogalactan was visualized by staining with 10 lg/mL SBA conjugated with Fluorescein (FL-1011–2, Vector Labs, USA) for 30 min. Chitin detection was performed by staining with 10 mg/ ml WGA conjugated with Alexa Fluor 350 (Invitrogen) for 30 min. For invasive hyphae staining, conidial suspensions were dropped onto barley leaves and incubated under high humidity. .. At 30 hpi, the conidial droplets were treated with SBA with Fluorescein or WGA and observed after an additional 30 min. Stained samples were examined under a confocal laser scanning microscope (Leica SP8, Leica Microsystems, Germany) to assess the distribution and changes of these components during M. oryzae development.

    Incubation:

    Article Title: Selectively targeting UDP-glucose 4-epimerase MoUGE1 for controlling rice blast disease.
    Article Snippet: Stained germinating conidia and appressoria were observed and imaged using a fluorescence microscope (Nikon Ni90, Japan). .. During various developmental stages of M. oryzae, galactosaminogalactan was visualized by staining with 10 lg/mL SBA conjugated with Fluorescein (FL-1011–2, Vector Labs, USA) for 30 min. Chitin detection was performed by staining with 10 mg/ ml WGA conjugated with Alexa Fluor 350 (Invitrogen) for 30 min. For invasive hyphae staining, conidial suspensions were dropped onto barley leaves and incubated under high humidity. .. At 30 hpi, the conidial droplets were treated with SBA with Fluorescein or WGA and observed after an additional 30 min. Stained samples were examined under a confocal laser scanning microscope (Leica SP8, Leica Microsystems, Germany) to assess the distribution and changes of these components during M. oryzae development.

    Article Title: PAX gene expression in autosomal dominant polycystic kidney disease contributes to cyst expansion and regulates a gene network associated with cyst growth.
    Article Snippet: .. After blocking in animal-free blocking buffer (Vector Laboratories, SP-5035), sections were incubated with primary antibodies (rabbit monoclonal anti-PAX2 (1:30; Abcam, ab79389), rabbit polyclonal anti-PAX8 (1:100; Biopat, PA 030), and anti-LRP2/megalin (1:200; Abcam, ab76969)), Alexa Fluor 594-conjugated goat anti-rabbit IgG (H + L) secondary antibody (1:200; ThermoFisher, A11012), and FITC-conjugated lectins (Lotus Tetragonolobus lectin (LTL; 1:50; ThermoFisher, L32480) and soybean agglutinin (SBA; 1:200; Vector Laboratories, FL-10112)) sequentially. .. Slides were mounted using ProLong Diamond Antifade with DAPI (ThermoFisher, P36965).

    Article Title: Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in bacteria.
    Article Snippet: Template DNA for bacterial OSTs was codon optimized and obtained as double-stranded DNA fragments known as gBlocks (Integrated DNA Technologies). .. Library transformants were replicated onto a nitrocellulose transfer membrane (BioRad, 0.45 μm), which were then placed colony-side-up on a second nitrocellulose transfer membrane and incubated at 30 °C for 18 h. The nitrocellulose transfermembranes were washed in Tris-buffered saline (TBS) for 10min, blocked in 5% bovine serum albumin for 30min, and probed for 1 h with fluorescein-labeled soybean agglutinin (SBA, Vector Laboratories, Cat # FL-1011, 1:500 dilution) and Alexa Fluor 647 (AF647)-conjugated anti-His antibody (R&D Systems, Cat # IC0501R, 1:2000 dilution) following themanufacturer’s instructions. ..

    Article Title: Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in bacteria
    Article Snippet: .. Library transformants were replicated onto a nitrocellulose transfer membrane (BioRad, 0.45 μm), which were then placed colony-side-up on a second nitrocellulose transfer membrane and incubated at 30 °C for 18 h. The nitrocellulose transfer membranes were washed in Tris-buffered saline (TBS) for 10 min, blocked in 5% bovine serum albumin for 30 min, and probed for 1 h with fluorescein-labeled soybean agglutinin (SBA, Vector Laboratories, Cat # FL-1011, 1:500 dilution) and Alexa Fluor 647 (AF647)-conjugated anti-His antibody (R&D Systems, Cat # IC0501R, 1:2000 dilution) following the manufacturer’s instructions. ..

    Blocking Assay:

    Article Title: PAX gene expression in autosomal dominant polycystic kidney disease contributes to cyst expansion and regulates a gene network associated with cyst growth.
    Article Snippet: .. After blocking in animal-free blocking buffer (Vector Laboratories, SP-5035), sections were incubated with primary antibodies (rabbit monoclonal anti-PAX2 (1:30; Abcam, ab79389), rabbit polyclonal anti-PAX8 (1:100; Biopat, PA 030), and anti-LRP2/megalin (1:200; Abcam, ab76969)), Alexa Fluor 594-conjugated goat anti-rabbit IgG (H + L) secondary antibody (1:200; ThermoFisher, A11012), and FITC-conjugated lectins (Lotus Tetragonolobus lectin (LTL; 1:50; ThermoFisher, L32480) and soybean agglutinin (SBA; 1:200; Vector Laboratories, FL-10112)) sequentially. .. Slides were mounted using ProLong Diamond Antifade with DAPI (ThermoFisher, P36965).

    Membrane:

    Article Title: Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in bacteria.
    Article Snippet: Template DNA for bacterial OSTs was codon optimized and obtained as double-stranded DNA fragments known as gBlocks (Integrated DNA Technologies). .. Library transformants were replicated onto a nitrocellulose transfer membrane (BioRad, 0.45 μm), which were then placed colony-side-up on a second nitrocellulose transfer membrane and incubated at 30 °C for 18 h. The nitrocellulose transfermembranes were washed in Tris-buffered saline (TBS) for 10min, blocked in 5% bovine serum albumin for 30min, and probed for 1 h with fluorescein-labeled soybean agglutinin (SBA, Vector Laboratories, Cat # FL-1011, 1:500 dilution) and Alexa Fluor 647 (AF647)-conjugated anti-His antibody (R&D Systems, Cat # IC0501R, 1:2000 dilution) following themanufacturer’s instructions. ..

    Article Title: Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in bacteria
    Article Snippet: .. Library transformants were replicated onto a nitrocellulose transfer membrane (BioRad, 0.45 μm), which were then placed colony-side-up on a second nitrocellulose transfer membrane and incubated at 30 °C for 18 h. The nitrocellulose transfer membranes were washed in Tris-buffered saline (TBS) for 10 min, blocked in 5% bovine serum albumin for 30 min, and probed for 1 h with fluorescein-labeled soybean agglutinin (SBA, Vector Laboratories, Cat # FL-1011, 1:500 dilution) and Alexa Fluor 647 (AF647)-conjugated anti-His antibody (R&D Systems, Cat # IC0501R, 1:2000 dilution) following the manufacturer’s instructions. ..

    Saline:

    Article Title: Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in bacteria.
    Article Snippet: Template DNA for bacterial OSTs was codon optimized and obtained as double-stranded DNA fragments known as gBlocks (Integrated DNA Technologies). .. Library transformants were replicated onto a nitrocellulose transfer membrane (BioRad, 0.45 μm), which were then placed colony-side-up on a second nitrocellulose transfer membrane and incubated at 30 °C for 18 h. The nitrocellulose transfermembranes were washed in Tris-buffered saline (TBS) for 10min, blocked in 5% bovine serum albumin for 30min, and probed for 1 h with fluorescein-labeled soybean agglutinin (SBA, Vector Laboratories, Cat # FL-1011, 1:500 dilution) and Alexa Fluor 647 (AF647)-conjugated anti-His antibody (R&D Systems, Cat # IC0501R, 1:2000 dilution) following themanufacturer’s instructions. ..

    Article Title: Discovery of a single-subunit oligosaccharyltransferase that enables glycosylation of full-length IgG antibodies in bacteria
    Article Snippet: .. Library transformants were replicated onto a nitrocellulose transfer membrane (BioRad, 0.45 μm), which were then placed colony-side-up on a second nitrocellulose transfer membrane and incubated at 30 °C for 18 h. The nitrocellulose transfer membranes were washed in Tris-buffered saline (TBS) for 10 min, blocked in 5% bovine serum albumin for 30 min, and probed for 1 h with fluorescein-labeled soybean agglutinin (SBA, Vector Laboratories, Cat # FL-1011, 1:500 dilution) and Alexa Fluor 647 (AF647)-conjugated anti-His antibody (R&D Systems, Cat # IC0501R, 1:2000 dilution) following the manufacturer’s instructions. ..



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    Image Search Results


    Heat map displaying lectin binding to selected sialylated and sulfated structures . The heat map was generated using the GLAD tool and represents the relative fluorescent units (RFU) normalized to 70,000 as the maximum value. Relevant epitopes are shown, structures including either Neu5Ac or Neu5Gc as terminal sialic acid or different linkages are indicated with a slash. For complete structures, please refer to .

    Journal: The Journal of Biological Chemistry

    Article Title: Enzymatic tailoring of anionic glycans for characterizing lectin and antibody specificities in a microarray format

    doi: 10.1016/j.jbc.2025.110915

    Figure Lengend Snippet: Heat map displaying lectin binding to selected sialylated and sulfated structures . The heat map was generated using the GLAD tool and represents the relative fluorescent units (RFU) normalized to 70,000 as the maximum value. Relevant epitopes are shown, structures including either Neu5Ac or Neu5Gc as terminal sialic acid or different linkages are indicated with a slash. For complete structures, please refer to .

    Article Snippet: Biotinylated lectins concanavalin A (ConA, 5 mg/ml), Erythrina cristagalli lectin (ECL, 5 mg/ml), Griffonia simplicifolia isolectin B4 (GSL-I, 0.5 mg/ml) and II (GSL-II, 2 mg/ml), Lens culinaris lectin (LCA, 5 mg/ml), Maackia amurensis lectin I and II (MAA-I, 2 mg/ml; MAA-II, 1 mg/ml), peanut lectin (PNA 5 mg/ml), Ricinus communis lectin I (RCA, 5 mg/ml), Sambucus nigra /Elderberry Bark lectin (SNA, 2 mg/ml), Vicia villosa lectin (VVA, 2 mg/ml), Wisteria floribunda agglutinin (WFA, 2 mg/ml) and wheat germ agglutinin (WGA, 5 mg/ml), as well as fluorescein-labeled soybean lectin (SBA, 2 mg/ml) were purchased from Vector Laboratories.

    Techniques: Binding Assay, Generated

    Heat map displaying Gal-specific lectin binding to all structures . The heat map was generated using the GLAD tool and represents the relative fluorescent units (RFU) normalized to 70,000 as the maximum value. Terminal modifications (of either one or both antennae) are shown. Unmodified β4Gal is indicated as x (no modification) while free terminal β3Gal is marked (compounds 21 and 38 ). For complete structures please refer to .

    Journal: The Journal of Biological Chemistry

    Article Title: Enzymatic tailoring of anionic glycans for characterizing lectin and antibody specificities in a microarray format

    doi: 10.1016/j.jbc.2025.110915

    Figure Lengend Snippet: Heat map displaying Gal-specific lectin binding to all structures . The heat map was generated using the GLAD tool and represents the relative fluorescent units (RFU) normalized to 70,000 as the maximum value. Terminal modifications (of either one or both antennae) are shown. Unmodified β4Gal is indicated as x (no modification) while free terminal β3Gal is marked (compounds 21 and 38 ). For complete structures please refer to .

    Article Snippet: Biotinylated lectins concanavalin A (ConA, 5 mg/ml), Erythrina cristagalli lectin (ECL, 5 mg/ml), Griffonia simplicifolia isolectin B4 (GSL-I, 0.5 mg/ml) and II (GSL-II, 2 mg/ml), Lens culinaris lectin (LCA, 5 mg/ml), Maackia amurensis lectin I and II (MAA-I, 2 mg/ml; MAA-II, 1 mg/ml), peanut lectin (PNA 5 mg/ml), Ricinus communis lectin I (RCA, 5 mg/ml), Sambucus nigra /Elderberry Bark lectin (SNA, 2 mg/ml), Vicia villosa lectin (VVA, 2 mg/ml), Wisteria floribunda agglutinin (WFA, 2 mg/ml) and wheat germ agglutinin (WGA, 5 mg/ml), as well as fluorescein-labeled soybean lectin (SBA, 2 mg/ml) were purchased from Vector Laboratories.

    Techniques: Binding Assay, Generated, Modification

    Fluorescence microscopy and flow cytometry to assess surface display of the Campylobacter heptasaccharide in Salmonella Typhimurium 82/6915 ΔaroA::pgl . (A) Fluorescence microscopy of OD 600 = 0.3 overnight fixed cultures of Salmonella Typhimurium 82/6915 ΔaroA::pgl and Salmonella Typhimurium 82/6915 or OD 600 = 0.3 48 h cultures of C. hepaticus HV10 T (Panel A) were blocked and stained with Gal N Ac-recognising lectin SBA conjugated to fluorescein or FITC anti- Salmonella antibody. Samples nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). (B) Flow cytometric analysis was acquired and analyzed on a Beckman Cytoflex using CytExpert 2.5 software. The equivalent of OD 600 = 0.3 overnight fixed cultures of Salmonella Typhimurium 82/6915 ΔaroA::pgl and Salmonella Typhimurium 82/6915 were blocked and then left unstained (orange and green, respectively) or probed with either SBA-fluorescein (dark blue and red, respectively) or FITC anti- Salmonella antibody (light blue and purple, respectively). Stained samples were diluted 1/1000 in PBS, and data were presented as histograms. A total of 10,000 events were analyzed for each sample.

    Journal: Frontiers in Veterinary Science

    Article Title: Immunological and pathobiological characteristics of a novel live Salmonella Typhimurium-vectored Campylobacter vaccine candidate for layer chickens

    doi: 10.3389/fvets.2025.1518231

    Figure Lengend Snippet: Fluorescence microscopy and flow cytometry to assess surface display of the Campylobacter heptasaccharide in Salmonella Typhimurium 82/6915 ΔaroA::pgl . (A) Fluorescence microscopy of OD 600 = 0.3 overnight fixed cultures of Salmonella Typhimurium 82/6915 ΔaroA::pgl and Salmonella Typhimurium 82/6915 or OD 600 = 0.3 48 h cultures of C. hepaticus HV10 T (Panel A) were blocked and stained with Gal N Ac-recognising lectin SBA conjugated to fluorescein or FITC anti- Salmonella antibody. Samples nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). (B) Flow cytometric analysis was acquired and analyzed on a Beckman Cytoflex using CytExpert 2.5 software. The equivalent of OD 600 = 0.3 overnight fixed cultures of Salmonella Typhimurium 82/6915 ΔaroA::pgl and Salmonella Typhimurium 82/6915 were blocked and then left unstained (orange and green, respectively) or probed with either SBA-fluorescein (dark blue and red, respectively) or FITC anti- Salmonella antibody (light blue and purple, respectively). Stained samples were diluted 1/1000 in PBS, and data were presented as histograms. A total of 10,000 events were analyzed for each sample.

    Article Snippet: The blocking solution was discarded, and cells were incubated for 1 h in the dark with either 25 μg/mL SBA fluorescein (Vector Laboratories) or 25 μg/mL FITC anti- Salmonella antibody (Abcam) diluted in PBST (PBS plus 0.1% Tween 20) containing 1% BSA.

    Techniques: Fluorescence, Microscopy, Flow Cytometry, Staining, Software