sba conjugated with fluorescein  (Vector Laboratories)

Journal: The Journal of Biological Chemistry

Article Title: Enzymatic tailoring of anionic glycans for characterizing lectin and antibody specificities in a microarray format

doi: 10.1016/j.jbc.2025.110915

Figure Lengend Snippet: Heat map displaying lectin binding to selected sialylated and sulfated structures . The heat map was generated using the GLAD tool and represents the relative fluorescent units (RFU) normalized to 70,000 as the maximum value. Relevant epitopes are shown, structures including either Neu5Ac or Neu5Gc as terminal sialic acid or different linkages are indicated with a slash. For complete structures, please refer to .

Article Snippet: Biotinylated lectins concanavalin A (ConA, 5 mg/ml), Erythrina cristagalli lectin (ECL, 5 mg/ml), Griffonia simplicifolia isolectin B4 (GSL-I, 0.5 mg/ml) and II (GSL-II, 2 mg/ml), Lens culinaris lectin (LCA, 5 mg/ml), Maackia amurensis lectin I and II (MAA-I, 2 mg/ml; MAA-II, 1 mg/ml), peanut lectin (PNA 5 mg/ml), Ricinus communis lectin I (RCA, 5 mg/ml), Sambucus nigra /Elderberry Bark lectin (SNA, 2 mg/ml), Vicia villosa lectin (VVA, 2 mg/ml), Wisteria floribunda agglutinin (WFA, 2 mg/ml) and wheat germ agglutinin (WGA, 5 mg/ml), as well as fluorescein-labeled soybean lectin (SBA, 2 mg/ml) were purchased from Vector Laboratories.

Techniques: Binding Assay, Generated

Journal: The Journal of Biological Chemistry

Article Title: Enzymatic tailoring of anionic glycans for characterizing lectin and antibody specificities in a microarray format

doi: 10.1016/j.jbc.2025.110915

Figure Lengend Snippet: Heat map displaying Gal-specific lectin binding to all structures . The heat map was generated using the GLAD tool and represents the relative fluorescent units (RFU) normalized to 70,000 as the maximum value. Terminal modifications (of either one or both antennae) are shown. Unmodified β4Gal is indicated as x (no modification) while free terminal β3Gal is marked (compounds 21 and 38 ). For complete structures please refer to .

Article Snippet: Biotinylated lectins concanavalin A (ConA, 5 mg/ml), Erythrina cristagalli lectin (ECL, 5 mg/ml), Griffonia simplicifolia isolectin B4 (GSL-I, 0.5 mg/ml) and II (GSL-II, 2 mg/ml), Lens culinaris lectin (LCA, 5 mg/ml), Maackia amurensis lectin I and II (MAA-I, 2 mg/ml; MAA-II, 1 mg/ml), peanut lectin (PNA 5 mg/ml), Ricinus communis lectin I (RCA, 5 mg/ml), Sambucus nigra /Elderberry Bark lectin (SNA, 2 mg/ml), Vicia villosa lectin (VVA, 2 mg/ml), Wisteria floribunda agglutinin (WFA, 2 mg/ml) and wheat germ agglutinin (WGA, 5 mg/ml), as well as fluorescein-labeled soybean lectin (SBA, 2 mg/ml) were purchased from Vector Laboratories.

Techniques: Binding Assay, Generated, Modification

Journal: Frontiers in Veterinary Science

Article Title: Immunological and pathobiological characteristics of a novel live Salmonella Typhimurium-vectored Campylobacter vaccine candidate for layer chickens

doi: 10.3389/fvets.2025.1518231

Figure Lengend Snippet: Fluorescence microscopy and flow cytometry to assess surface display of the Campylobacter heptasaccharide in Salmonella Typhimurium 82/6915 ΔaroA::pgl . (A) Fluorescence microscopy of OD 600 = 0.3 overnight fixed cultures of Salmonella Typhimurium 82/6915 ΔaroA::pgl and Salmonella Typhimurium 82/6915 or OD 600 = 0.3 48 h cultures of C. hepaticus HV10 T (Panel A) were blocked and stained with Gal N Ac-recognising lectin SBA conjugated to fluorescein or FITC anti- Salmonella antibody. Samples nuclei were counterstained with 4′,6-diamidino-2-phenylindole (DAPI). (B) Flow cytometric analysis was acquired and analyzed on a Beckman Cytoflex using CytExpert 2.5 software. The equivalent of OD 600 = 0.3 overnight fixed cultures of Salmonella Typhimurium 82/6915 ΔaroA::pgl and Salmonella Typhimurium 82/6915 were blocked and then left unstained (orange and green, respectively) or probed with either SBA-fluorescein (dark blue and red, respectively) or FITC anti- Salmonella antibody (light blue and purple, respectively). Stained samples were diluted 1/1000 in PBS, and data were presented as histograms. A total of 10,000 events were analyzed for each sample.

Article Snippet: The blocking solution was discarded, and cells were incubated for 1 h in the dark with either 25 μg/mL SBA fluorescein (Vector Laboratories) or 25 μg/mL FITC anti- Salmonella antibody (Abcam) diluted in PBST (PBS plus 0.1% Tween 20) containing 1% BSA.

Techniques: Fluorescence, Microscopy, Flow Cytometry, Staining, Software